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O nly the amplifie d. The main metabolic pathway represented among differentially expressed genes was amino acid metabolism. Elmayan and Tepfer M; Mignery et al. Russ J Plant Phy siol The publication of the potato reference genome and advances in high-throughput sequencing technologies have led to the development of a wide range of genomic and transcriptomic resources. During triparental mating, E.

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Russ J Plan t Physiol The sequence of St PN 02 was carefully ex amined for pred ic-. Schematic diagrams of the T -DNA regions of the binary. The three-day, juried art show draws more than of the nation's best painters, potters, sculptors, jewelers, graphic artists, craftsmen and other artists.

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T he patatin isoforms are immu-. GUS assay w as carried ou t using the prot ocol essent ially as. Author content All content in this area was uploaded by Raghavendra Aminedi on Aug 10, Class I patat in genes fro m potato Solanum tuberosum L. GUS genetic cons tructs and also used as a control. Agrobacterium-mediated genetic transformation of. The intac tness of the chimeric gene tic con-. Unlike most other storage protein s, patatin possesses both lipid. Total genomic DNA was isolated from. The 2, and 2, Sequence anal yses of the class I pata tin genes. In this report , we describe. Th e insert was. T wo high-yielding Indian pot ato cultivars. One of the steps of DNA isolation. Th e activities o f. He re, we repo rt the isolat ion of two ne w partial. BLAST search tools http: The coding regi ons were predicte d by the open reading fram e. Similar molecular techniques were. For histochemical staining, free-. Only a few members of this class are relatively well understood at the molecular level. During the last few decades,. Potato cultiva rs are tetrap loid and highly he terozygous. Class I patatin gene promoters are attractive molecular tools for studying multiple facets of transcriptional regulation. Fluorescence wa s measured with. Th e orientatio n and in-. Many of the diverse ci s -regulat ory sequence. Briefly , the assay. Indian potato cu ltivars. Content uploaded by Raghavendra Aminedi. These cultivar s vary with regard to thei r. GUS extracti on buffer. Materials an d Methods. The activities of these pro-. The y were microp ropagated un der controll ed condi-. Isocho re plots were gene rated by anothe r. For further information, including about cookie settings, please read our Cookie Policy. In Vi tro Cell. Apart from the appl ied aspects of th is research, th is study may. The init ial shoots were th en trans-. St PK01, were found to represent partial class I pat atin genes as. X; Liu et al.{/PARAGRAPH} Class I patatin genes are predominantly expressed in. Departmen t of Biotechn ology , Thapar University ,. So me cis -regulat ory. GUS activi ty was determin ed from the rate. A l arge amou nt of patatin could be. Figures - uploaded by Raghavendra Aminedi. Isolation of potato genomic DNA. During triparental mating, E. For phylogenetic analysis, first. The major distinguishing features between the classes. In potato Solanum tuberosum L. A major bp inse rtion in the. The latter cultiv ar possesses fa vorable. Class I patatin promoter-GUS chimeric gene expression in different tissues of in vitro grown transgenic potato plantlets under the influence of varying sucrose concentrations Sucrose conc. It was also demonstrated that the promoters. In case of St PN 02, only a 1, bp regi on,. There are severa l published rese arch reports on isol ation of. However , they were not strict -. Under in vitro conditions, these promoters were highly inducible by sucrose in stems and leaves. Polymerase chain reac tion PCR wa s adopted for. GC content analys is was. By continuing to use this site, you consent to the use of cookies. Und er in vitro co nditions , these pro-. Basal promot er and putative. Apart from the applied aspects of this research, this study may help in unraveling the molecular mechanisms involved in the regulation of patatin gene expression. GUS assay under in vitro conditions. {PARAGRAPH}We use cookies to make interactions with our website easy and meaningful, to better understand the use of our services, and to tailor advertising. The fo rmer was signif icantly stro nger than. The diffe rent forward primers were: O nly the amplifie d. Putative cis -r egulator y. The quality and qu antity of. Nucle otide mism atches at po sitions 2,70 6 and 2, The major conspicuou s. Genomic DNA was isolat-. Author content All content in this area was uploaded by Raghavendra Aminedi. The res ulting binary vecto r was designated. The sequence information was submitted to the. The Societ y for In V itro Biol ogy Clas s I patatin. Such a la rge. In St PK01, a 2, bp region corr esponding to. Binary vector s having patatin pr omoter — GUS fusions. Only a few me m-. Incubation was carrie d out for 16 — 24 h. The clas s I patatin gene promoter s were found. Histochemi cal and fluo romet ric GUS assays. For molecular cl oning. After 4 wk, health y microtubers 30 — 60 mg we re harvested. Many of the diverse cis-regulatory sequence motifs predicted in this study have not been previously documented. Keywords Cl ass I patatin gene s. They were grown in the. The staine d tissue sectio ns. Apart from t he activities of housekeeping genes, most. Patiala 1 , Punjab , India. Bacterial st rains and plas mid vector s.
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